Composite

Part:BBa_K1602056

Designed by: Leon Kraus, Jonathan Pletzer-Zelgert, Jonas Sindlinger, Carmen Sakas Gandullo, Sebastian Holzner, Elena Tonchevska, Mirjam Haun   Group: iGEM15_TU_Darmstadt   (2015-09-16)

B0034-yjhG-B0034-yqhD

This composite represents the D-xylonic acid dehydratase yjhG and the aldehyde reductase YqhD in one plasmid. Both genes have the strong ribosome binding site BBa_B0034 before their sequence.

Usage

This part is a composite of two coding genes and one strong RBS per gene (BBa_B0034).

  • yjhG
  • YqhD
  • RBS-B0034
https://static.igem.org/mediawiki/parts/c/c3/TU_Darmstadt_plasmid-schema_B0034-yjhG-B0034-yqhD.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1921
    Illegal AgeI site found at 2436
    Illegal AgeI site found at 2630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 179
    Illegal SapI.rc site found at 3093
[edit]
Categories
Parameters
None